Moving ahead with the global revolution in diagnostic microbiology, HiMedia presents HiCrome Mueller Hinton Agar (M2010) with dual advantage as another jewel in the crown of HiCrome series.

Chromogenic Identification + Antimicrobial Susceptibility

  • Chromogenic differentiation of various urinary tract pathogens
  • Simultaneous detection of susceptibility to antimicrobials
  • Rapid and reliable results in 24 hours as compared to traditional method, which takes 48 hours for organism identification and antimicrobial susceptibility

Microorganism Identification Based on Colony Colors

  • Pink to purple : Escherichia coli 
  • Metallic blue : Enterobacter spp. or Klebsiella spp. 
  • Blue : Enterococcus faecalis
  • Colorless to golden yellow : Staphylococcus aureus
  • Brown coloration : Proteus spp. 
  • Colorless : Yeast spp.

Mueller Hinton Agar is used as a test medium for antimicrobial susceptibility testing, and is recommended by CLSI. Kirby-Bauer et al. (1966) recommended this medium for performing antibiotic susceptibility tests using a single disc of high concentration. WHO Committee on Standardization of Susceptibility Testing has accepted Mueller Hinton Agar for determining the susceptibility of microorganisms because of its reproducibility. The ingredients are selected for low thymine and thymidine content as determined by MIC values for E. faecalis with sulfamethoxazole trimethoprim. Calcium and magnesium ion concentrations are adjusted to provide the amounts recommended by CLSI to give the correct MIC values with aminoglycosides and Pseudomonas aeruginosa.

HiCrome Mueller Hinton Agar is used for presumptive identification and susceptibility testing of bacteria directly from clinical samples. It provides high medical value to critical care cases, where faster treatment decisions are expected, as it provides both identification and susceptibility results within 24 hours.

Chromogenic mixture incorporated helps in color differentiation. One of the chromogenic substrates is cleaved by ß-glucosidase possessed by Enterococci resulting in formation of blue colonies. E. coli produces pink to purple colonies due to the enzyme ß-D-galactosidase that cleaves the other chromogenic substrate. S. aureus produces colorless colonies. P. aeruginosa produces greenish pigmentation. Klebsiella and Enterobacter species produce metallic-blue-colored colonies. Colonies of Proteus, Morganella, and Providencia species appear brown. This medium can be employed in screening urinary tract pathogens, wherein organisms can be differentiated based on color and simultaneously the antibiotic sensitivity can be determined.

Product Listing. Dehydrated culture media – powdered, granulated, encapsulated, HiVeg, and HiCynth; ready prepared media; antimicrobial susceptibility systems; lyophilized ATCC cultures; stains and indicators; and animal and vegetable hydrolysates.

 


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